%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Le, Q.V.
%A Bogusz, Didier
%A Gherbi, H.
%A Lappartient, A.
%A Duhoux, Emile
%A Franche, C.
%T Agrobacterium tumefaciens gene transfer to Casuarina glauca : a tropical nitrogen-fixing tree
%D 1996
%L fdi:010011871
%G ENG
%J Plant Science
%@ 0168-9452
%K AMELIORATION DES PLANTES ; AMELIORATION GENETIQUE ; ARBRE FORESTIER ; TRANSFERT DE GENE ; BACTERIE ; ACTIVITE ENZYMATIQUE
%K PLANTE TRANSGENIQUE ; BIOLISTIQUE
%P 57-69
%R 10.1016/0168-9452(96)04386-5
%U https://www.documentation.ird.fr/hor/fdi:010011871
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010011871.pdf
%V 118
%W Horizon (IRD)
%X Transgenic calli of the tropical tree #Casuarina glauca$ were produced using #Agrobacterium tumefaciens$-mediated gene transfer. Hypocotyls, cotyledons and epicotyls were excised from 30-60-day old #Casuarina$ seedlings and cocultivated with #Agrobacterium$ strain C58C1(pGV2260) containing the binary vector BIN19GUSINT. Transformed calli were selected on nutrient medium supplemented with 0,5 microM NAA, 2,5 microM BA and 50 mg/l kanamycin. Some of the factors influencing T-DNA transfer to #C. glauca$ explants were studied. Optimal transformation rates were obtained when explants from 45-day old seedlings were cocultivated for 3 days in the presence of 25 microM acetosyringone. Transgenic buds differentiated on 10% of the calli grown on transformed epicotyls. Evidence for genetic transformation was obtained by beta-glucuronidase assay, PCR and Southern hybridization. (Résumé d'auteur)
%$ 076AMEPLA04