@article{fdi:010011871,
  title = {{A}grobacterium tumefaciens gene transfer to {C}asuarina glauca : a tropical nitrogen-fixing tree},
  author = {{L}e, {Q}.{V}. and {B}ogusz, {D}idier and {G}herbi, {H}. and {L}appartient, {A}. and {D}uhoux, {E}mile and {F}ranche, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ransgenic calli of the tropical tree #{C}asuarina glauca$ were produced using #{A}grobacterium tumefaciens$-mediated gene transfer. {H}ypocotyls, cotyledons and epicotyls were excised from 30-60-day old #{C}asuarina$ seedlings and cocultivated with #{A}grobacterium$ strain {C}58{C}1(p{GV}2260) containing the binary vector {BIN}19{GUSINT}. {T}ransformed calli were selected on nutrient medium supplemented with 0,5 micro{M} {NAA}, 2,5 micro{M} {BA} and 50 mg/l kanamycin. {S}ome of the factors influencing {T}-{DNA} transfer to #{C}. glauca$ explants were studied. {O}ptimal transformation rates were obtained when explants from 45-day old seedlings were cocultivated for 3 days in the presence of 25 micro{M} acetosyringone. {T}ransgenic buds differentiated on 10% of the calli grown on transformed epicotyls. {E}vidence for genetic transformation was obtained by beta-glucuronidase assay, {PCR} and {S}outhern hybridization. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{AMELIORATION} {DES} {PLANTES} ; {AMELIORATION} {GENETIQUE} ; {ARBRE} {FORESTIER} ; {TRANSFERT} {DE} {GENE} ; {BACTERIE} ; {ACTIVITE} {ENZYMATIQUE} ; {PLANTE} {TRANSGENIQUE} ; {BIOLISTIQUE}},
  booktitle = {},
  journal = {{P}lant {S}cience},
  volume = {118},
  numero = {},
  pages = {57--69},
  ISSN = {0168-9452},
  year = {1996},
  DOI = {10.1016/0168-9452(96)04386-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010011871},
}