Le Q.V., Bogusz Didier, Gherbi H., Lappartient A., Duhoux Emile, Franche C. (1996). Agrobacterium tumefaciens gene transfer to Casuarina glauca : a tropical nitrogen-fixing tree. Plant Science, 118, p. 57-69. ISSN 0168-9452.
Titre du document
Agrobacterium tumefaciens gene transfer to Casuarina glauca : a tropical nitrogen-fixing tree
Année de publication
1996
Type de document
Article
Auteurs
Le Q.V., Bogusz Didier, Gherbi H., Lappartient A., Duhoux Emile, Franche C.
Source
Plant Science, 1996,
118, p. 57-69 ISSN 0168-9452
Transgenic calli of the tropical tree Casuarina glauca were produced using Agrobacterium tumefaciens-mediated gene transfer. Hypocotyls, cotyledons and epicotyls were excised from 30-60-day old Casuarina seedlings and cocultivated with Agrobacterium strain C58C1(pGV2260) containing the binary vector BIN19GUSINT. Transformed calli were selected on nutrient medium supplemented with 0,5 microM NAA, 2,5 microM BA and 50 mg/l kanamycin. Some of the factors influencing T-DNA transfer to C. glauca explants were studied. Optimal transformation rates were obtained when explants from 45-day old seedlings were cocultivated for 3 days in the presence of 25 microM acetosyringone. Transgenic buds differentiated on 10% of the calli grown on transformed epicotyls. Evidence for genetic transformation was obtained by beta-glucuronidase assay, PCR and Southern hybridization. (Résumé d'auteur)
