%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Thouvenel, Jean-Claude
%A Abol-Ela, S.
%A Sewify, G.H.
%A El-Hariry, M.
%A Hussein, A.
%A Ammar, E.D.
%T Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt
%D 1996
%L fdi:010011007
%G ENG
%J Bulletin of Faculty of Agriculture of University of Cairo
%K PATHOLOGIE VEGETALE ; VIRUS ; SEROLOGIE ; PROTEINE
%K MAIZE YELLOW STRIPE VIRUS
%P 179-190
%U https://www.documentation.ird.fr/hor/fdi:010011007
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010011007.pdf
%V 47
%W Horizon (IRD)
%X The nucleoprotein and non-capsid protein of maize yellow stripe virus (MYSV) were purified from naturally or experimentally infected maize or sorghum plants. In SDS-PAGE, the apparent molecular weight of the nucleoprotein was 35,6 KD, and that of the non-capsid protein was 14,7 KD. Similar to tenuiviruses, the nucleoprotein of MYSV was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. Following a 2-day acquisition feeding period on MYSV-infected plants, vector leafhoppers (#Cicadulina chinai$) remained highly infective for 21 days. Antisera to the nucleoprotein and non-capsid protein of MYSV were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oat and the graminaceous weeds #Bromus wildenowii$, #Cenchrus biflorus$, #Dichanthium annulatum$, #Digitaria sanguinalis$, #Echinochloa colonum$, #Setaria viridis$ and #S. verticillata$. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers. (Résumé d'auteur)
%$ 076MALPLA03