@article{fdi:010011007, title = {{C}haracterization and serology of the leafhopper-borne maize yellow stripe virus in {E}gypt}, author = {{T}houvenel, {J}ean-{C}laude and {A}bol-{E}la, {S}. and {S}ewify, {G}.{H}. and {E}l-{H}ariry, {M}. and {H}ussein, {A}. and {A}mmar, {E}.{D}.}, editor = {}, language = {{ENG}}, abstract = {{T}he nucleoprotein and non-capsid protein of maize yellow stripe virus ({MYSV}) were purified from naturally or experimentally infected maize or sorghum plants. {I}n {SDS}-{PAGE}, the apparent molecular weight of the nucleoprotein was 35,6 {KD}, and that of the non-capsid protein was 14,7 {KD}. {S}imilar to tenuiviruses, the nucleoprotein of {MYSV} was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. {F}ollowing a 2-day acquisition feeding period on {MYSV}-infected plants, vector leafhoppers (#{C}icadulina chinai$) remained highly infective for 21 days. {A}ntisera to the nucleoprotein and non-capsid protein of {MYSV} were produced and used for detection of this virus in several host plants and vector leafhoppers in {E}gypt. {D}ot-blot and direct antigen coating ({DAC}) {ELISA} were used to detect {MYSV} in naturally or experimentally infected maize, wheat, barley, oat and the graminaceous weeds #{B}romus wildenowii$, #{C}enchrus biflorus$, #{D}ichanthium annulatum$, #{D}igitaria sanguinalis$, #{E}chinochloa colonum$, #{S}etaria viridis$ and #{S}. verticillata$. {D}ot-blot and {DAC}-{ELISA} were used also to detect {MYSV} in naturally or experimentally infective leafhoppers. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{PATHOLOGIE} {VEGETALE} ; {VIRUS} ; {SEROLOGIE} ; {PROTEINE} ; {MAIZE} {YELLOW} {STRIPE} {VIRUS}}, booktitle = {}, journal = {{B}ulletin of {F}aculty of {A}griculture of {U}niversity of {C}airo}, volume = {47}, numero = {}, pages = {179--190}, year = {1996}, URL = {https://www.documentation.ird.fr/hor/fdi:010011007}, }