Thouvenel Jean-Claude, Abol-Ela S., Sewify G.H., El-Hariry M., Hussein A., Ammar E.D. (1996). Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt. Bulletin of Faculty of Agriculture of University of Cairo, 47, p. 179-190.
Titre du document
Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt
Année de publication
1996
Type de document
Article
Auteurs
Thouvenel Jean-Claude, Abol-Ela S., Sewify G.H., El-Hariry M., Hussein A., Ammar E.D.
Source
Bulletin of Faculty of Agriculture of University of Cairo, 1996,
47, p. 179-190
The nucleoprotein and non-capsid protein of maize yellow stripe virus (MYSV) were purified from naturally or experimentally infected maize or sorghum plants. In SDS-PAGE, the apparent molecular weight of the nucleoprotein was 35,6 KD, and that of the non-capsid protein was 14,7 KD. Similar to tenuiviruses, the nucleoprotein of MYSV was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. Following a 2-day acquisition feeding period on MYSV-infected plants, vector leafhoppers (Cicadulina chinai) remained highly infective for 21 days. Antisera to the nucleoprotein and non-capsid protein of MYSV were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oat and the graminaceous weeds Bromus wildenowii, Cenchrus biflorus, Dichanthium annulatum, Digitaria sanguinalis, Echinochloa colonum, Setaria viridis and S. verticillata. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers. (Résumé d'auteur)
Plan de classement
Symptomatologie, diagnostic des agents pathogènes [076MALPLA03]
