%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Ohresser, M.
%A Borsa, Philippe
%A Delsert, C.
%T Intron-length polymorphism at the actin gene locus mac-1 : a genetic marker for population studies in the marine mussels Mytilus galloprovincialis Lmk. and M. edulis L.
%D 1997
%L fdi:010011003
%G ENG
%J Molecular Marine Biology and Biotechnology
%K INVERTEBRE AQUATIQUE ; POLYMORPHISME GENETIQUE ; TAXONOMIE
%N 2
%P 123-130
%U https://www.documentation.ird.fr/hor/fdi:010011003
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010011003.pdf
%V 6
%W Horizon (IRD)
%X A novel intron-length polymorphism at the actin gene locus mac-1 is here reported and used as a genetic marker for population studies in mussels of the genus #Mytilus$. Two closely related genes subsequently identified as alleles, mac-1a1, and mac-1b1, from a genomic library of #M. galloprovincialis$ were partially cloned and sequenced. They mainly differed from each other by a 65-bp insertion within their first intron. Polymerase chain reaction (PCR) primers were designed outside the insertion. The PCR analysis of 166 individual mussels from #M. galloprovincialis$ and #M. edulis$ populations revealed three size-classes of alleles or allelomorphs, two of which were of the expected sizes for mac-1a1 and mac-1b1. One allelomorph was absent from #M. edulis$ samples, although it was present at substantial frequencies in #M. galloprovincialis$ populations. The frequencies of the two other allelomorphs significantly differed between #M. galloprovincialis$ and #M. edulis$ populations. The comparison of six mac-1 intron sequences over 277 bp showed at once that allelomorphs encompassed alleles differing from one another by substantial numbers of mutations, and that identical alleles were present in both #M. galloprovincialis$ and #M. edulis$ individuals, a probable result of the recent introgression between the two species. (Résumé d'auteur)
%$ 034BIOINV03