@article{fdi:010011003, title = {{I}ntron-length polymorphism at the actin gene locus mac-1 : a genetic marker for population studies in the marine mussels {M}ytilus galloprovincialis {L}mk. and {M}. edulis {L}.}, author = {{O}hresser, {M}. and {B}orsa, {P}hilippe and {D}elsert, {C}.}, editor = {}, language = {{ENG}}, abstract = {{A} novel intron-length polymorphism at the actin gene locus mac-1 is here reported and used as a genetic marker for population studies in mussels of the genus #{M}ytilus$. {T}wo closely related genes subsequently identified as alleles, mac-1a1, and mac-1b1, from a genomic library of #{M}. galloprovincialis$ were partially cloned and sequenced. {T}hey mainly differed from each other by a 65-bp insertion within their first intron. {P}olymerase chain reaction ({PCR}) primers were designed outside the insertion. {T}he {PCR} analysis of 166 individual mussels from #{M}. galloprovincialis$ and #{M}. edulis$ populations revealed three size-classes of alleles or allelomorphs, two of which were of the expected sizes for mac-1a1 and mac-1b1. {O}ne allelomorph was absent from #{M}. edulis$ samples, although it was present at substantial frequencies in #{M}. galloprovincialis$ populations. {T}he frequencies of the two other allelomorphs significantly differed between #{M}. galloprovincialis$ and #{M}. edulis$ populations. {T}he comparison of six mac-1 intron sequences over 277 bp showed at once that allelomorphs encompassed alleles differing from one another by substantial numbers of mutations, and that identical alleles were present in both #{M}. galloprovincialis$ and #{M}. edulis$ individuals, a probable result of the recent introgression between the two species. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{INVERTEBRE} {AQUATIQUE} ; {POLYMORPHISME} {GENETIQUE} ; {TAXONOMIE}}, booktitle = {}, journal = {{M}olecular {M}arine {B}iology and {B}iotechnology}, volume = {6}, numero = {2}, pages = {123--130}, year = {1997}, URL = {https://www.documentation.ird.fr/hor/fdi:010011003}, }