@article{fdi:010010396,
  title = {{O}ptimization of parameters for particle bombardment of embryogenic suspension cultures of cassava ({M}anihot esculenta {C}rantz) using computer image analysis},
  author = {{S}ch{\¨o}pke, {C}. and {T}aylor, {N}.{J}. and {C}arcamo, {R}. and {B}eachy, {R}.{N}. and {F}auquet, {C}laude},
  editor = {},
  language = {{ENG}},
  abstract = {{T}issue derived from embryonic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uid{A} gene, which codes for beta-glucuronidase ({GUS}). {A}fter 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological {GUS} assays. {C}ounting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. {A} reproducible counting method was established by optimizing {GUS} assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue. {T}he effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on {GUS} expression were investigated. {O}ptimal transient expression of the uid{A} gene was observed after bombardment at 1100 psi, with a particle size of 1 micrometer, an osmotic pretreatment and two bombardments per sample. {T}he highest number of blue spots observed was 2400 per square centimeter of bombarded tissue. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{AMELIORATION} {DES} {PLANTES} ; {AMELIORATION} {GENETIQUE} ; {EMBRYON} {ZYGOTIQUE} ; {CULTURE} {IN} {VITRO} ; {ANALYSE} {D}'{IMAGE} ; {BIOLISTIQUE}},
  booktitle = {},
  journal = {{P}lant {C}ell {R}eports},
  volume = {16},
  numero = {8},
  pages = {526--530},
  ISSN = {0721-7714},
  year = {1997},
  DOI = {10.1007/{BF}01142317},
  URL = {https://www.documentation.ird.fr/hor/fdi:010010396},
}