@article{fdi:010009566,
  title = {{A} rapid method for sequencing of r{RNA} gene(s) amplified by polymerase chain reaction using an automated {DNA} sequencer},
  author = {{D}wivedi, {P}.{P}. and {P}atel, {B}.{K}.{C}. and {R}ees, {G}.{N}. and {O}llivier, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{A} method for {DNA} sequencing of ribosomal {RNA} (r{RNA}) genes, amplified by polymerase chain reaction ({PCR}), using internal primers, designed on the basis of conserved regions of r{RNA} genes for determining a near complete sequence (99%) of the gene using an automated {DNA} sequencer ({A}pplied {B}iosystem {I}ncorporation, {USA}) is described. {T}he procedure is extremely rapid as cloning of the gene is not required for sequence determination. {I}n addition time consuming steps such as ethanol precipitation and hazardous steps such as phenol/chloroform extractions are excluded from the protocol for the purification of extension products after {T}aq cycle sequencing using the {ABI} dye terminator chemistry. {T}he method has been successfully used for sequencing of the 16{S} and 18{S} r{RNA} genes of microbes which includes six members of domain {B}acteria, one of domain {A}rchaea and one belonging to {E}ukarya domain. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{BIOLOGIE} {MOLECULAIRE} ; {GENE} ; {ARN} ; {METHODE} {D}'{ANALYSE} ; {PCR}.{REACTION} {DE} {POLYMERISATION} {EN} {CHAINE}},
  booktitle = {},
  journal = {{I}ndian {J}ournal of {M}icrobiology},
  volume = {36},
  numero = {},
  pages = {9--12},
  year = {1996},
  URL = {https://www.documentation.ird.fr/hor/fdi:010009566},
}