%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Kpémoua, K. %A Boher, Bernard %A Nicole, Michel %A Calatayud, Paul-André %A Geiger, Jean-Paul %T Cytochemistry of defense responses in cassava infected by Xanthomonas campestris pv. manihotis %D 1996 %L fdi:010008188 %G ENG %J Canadian Journal of Microbiology %@ 0008-4166 %K MALADIE DES PLANTES ; BACTERIE ; RELATION HOTE PARASITE ; RESISTANCE DE L'HOTE ; BIOCHIMIE ; COMPOSE PHENOLIQUE ; MICROSCOPIE ELECTRONIQUE %P 1131-1143 %R 10.1139/m96-145 %U https://www.documentation.ird.fr/hor/fdi:010008188 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010008188.pdf %V 42 %W Horizon (IRD) %X Stems of susceptible and resistant cassava plants have been cytologically investigated for their defense reactions to an aggressive strain of Xanthomonas campestris pv. manihotis. Histochemistry, in conjunction with gold cytochemistry, revealed that in susceptible and resistant plants, phloem and xylem parenchyma cells displayed a wide range of responses that limited the bacterial growth within the infected plants. Lignification and suberization associated with callose deposition were effective mechanisms that reinforced host barriers in the phloem. In the infected xylem, vessels were plugged by a material of pectic and (or) lignin-like origin. Flavonoids have been seen to be incorporated in secondary cell wall coatings. These reactions occurred at a higher intensity in the resistant plants. The number of phoem and xylem cells producing autofluorescent compounds was higher in infected resistant plants than in susceptible plants. Reactions have been observed in the resistant variety only, such as secretion of phenol-like molecules by tyloses and hyperplasic activity of phloem cells that compartmentalized bacterial lysis pockets, which are potent secondary inoculum sources.Key words: lignin, suberin, callose, phenol, tylose, flavonoid, pectin %$ 076MALPLA07