@article{fdi:010008184,
  title = {{H}uman plasmatic apolipoprotein {H} binds {H}uman {I}mmunodeficiency {V}irus type 1 and type 2 proteins},
  author = {{S}tefas, {E}lias and {R}ucheton, {M}arcel and {G}raafland, {H}. and {M}oynier, {M}. and {S}ompeyrac, {C}. and {B}ahraoui, {E}.{M}. and {V}eas, {F}rancisco},
  editor = {},
  language = {{ENG}},
  abstract = {{A}polipoprotein {H} (apo {H}), isolated from human plasma albumin solution, was shown to capture {HIV}-1-related antigens from antigen-positive sera ({HIV}-1 {AG}+) of {AIDS} patients, by using {HIV}-1-specific polyclonal antibodies. {I}n an enzyme-linked immunosorbent assay and ligand blot and dot assays, apo {H} was able to bind recombinant retroviral {HIV} antigens, especially {G}ag proteins p18 of {HIV}-1, p26 of {HIV}-2, and {E}nv gp160 of {HIV}-1. {B}inding was shown to be p{H} and {N}a{C}l dependent, with an optimum at acidic p{H} and low ionic strength. {S}pecificity was demonstrated by saturation of this binding and inhibition either by homologous competition or by specific antisera. {B}inding was also observed in cell line-harvested viral blotted proteins. {T}he mechanism of this apo {H}-polyspecific binding is discussed in relation to conformational changes due to the influence of lipids or detergents. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{SIDA} ; {AGENT} {PATHOGENE} ; {ANTIGENE} ; {AFFINITE} ; {PROTEINE} ; {APOLIPOPROTEINE} {H} ; {VIRUS} {HIV}-1 ; {VIRUS} {HIV}-2 ; {TECHNIQUE} {ELISA} ; {DEJECTION}},
  booktitle = {},
  journal = {{AIDS} {R}esearch and {H}uman {R}etroviruses},
  volume = {13},
  numero = {1},
  pages = {97--104},
  ISSN = {0889-2229},
  year = {1997},
  DOI = {10.1089/aid.1997.13.97},
  URL = {https://www.documentation.ird.fr/hor/fdi:010008184},
}