Wincker P., Telleria J., Bosseno Marie-France, Cardoso M.A., Marques P., Yaksic N., Aznar C., Liegeard M., Hontebeyrie M., Noireau François, Morel C.M., Brenière Simone Frédérique. (1996). PCR-based diagnosis for Chagas' disease in bolivian children living in an active transmission area : comparison with conventional serological and parasitological diagnosis. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 297. ISSN 0074-0276.
Titre du document
PCR-based diagnosis for Chagas' disease in bolivian children living in an active transmission area : comparison with conventional serological and parasitological diagnosis
Année de publication
1996
Auteurs
Wincker P., Telleria J., Bosseno Marie-France, Cardoso M.A., Marques P., Yaksic N., Aznar C., Liegeard M., Hontebeyrie M., Noireau François, Morel C.M., Brenière Simone Frédérique
Source
Memorias do Instituto Oswaldo Cruz, 1996,
91 (no spécial), p. 297 ISSN 0074-0276
A large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnosis methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buffy coat examination, 106 were detected by PCR (sensitivity : 93,8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, or IgG-, IgM-, and buffy coat -negative) only one case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections. (Résumé d'auteur)
Plan de classement
Epidémiologie des trypanosomiases [052GLOTRY02]
Descripteurs
MALADIE DE CHAGAS ; DIAGNOSTIC ; GENETIQUE ; SEROLOGIE ; ETUDE COMPARATIVE ; PCR.REACTION DE POLYMERISATION EN CHAINE ; IMMUNOGLOBULINE M
Description Géographique
BOLIVIE
Localisation
Fonds IRD [F B010007855] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010007855
