%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Guevara, A.G.
%A Taibi, A.
%A Billaut-Mulot, O.
%A Lemesre, Jean-Loup
%A Ouaissi, A.
%T Sera from Trypanosoma b. gambiense infected patients cross-react with a Trypanosoma cruzi recombinant protein
%D 1996
%L fdi:010007851
%G ENG
%J Memorias do Instituto Oswaldo Cruz
%@ 0074-0276
%K ANTIGENE ; CLONE ; TRYPANOSOMIASE HUMAINE
%K PCR.REACTION DE POLYMERISATION EN CHAINE ; PROTEINE RECOMBINANTE
%N no spécial
%P 255
%U https://www.documentation.ird.fr/hor/fdi:010007851
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010007851.pdf
%V 91
%W Horizon (IRD)
%X In previous studies, we and others have shown utility of a 24-kDa #Trypanosoma cruzi$ recombinant antigen (rTc24) for serological diagnosis of Chagas' disease. Also, this molecule has been proved useful to evaluate cure of chagasic patients who submit to specific treatment. However, in all the studies done so far, the 24-kDa protein was used as a fusion with a Gluthatione-S-transferase (GST) of #Schistosoma japonicum$, therefore, parallel assays to determine the anti-GST responses of all sera were required to deduce the GST noise in serological tests. Here, we show the subcloning by polymerase chain reaction of the cDNA encoding the #T. cruzi$ 24-kDa antigen in a vector system (pQE) allowing us to obtain Tc24 recombinant protein as a single molecule. The highly reactivity of chagasic sera from Colombia, Ecuador, Brazil and Bolivia in ELISA against the recombinant antigen is confirmed. However, sera from patients infected with African trypanosomes recognize rTc24 in ELISA and blot. The relevance of these findings in the context of Chagas' disease diagnosis and/or the relationship with African trypanosomes is analyzed. (Résumé d'auteur)
%$ 052GLOTRY02