Publications des scientifiques de l'IRD

Guevara A.G., Taibi A., Billaut-Mulot O., Lemesre Jean-Loup, Ouaissi A. (1996). Sera from Trypanosoma b. gambiense infected patients cross-react with a Trypanosoma cruzi recombinant protein. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 255. ISSN 0074-0276.

Titre du document
Sera from Trypanosoma b. gambiense infected patients cross-react with a Trypanosoma cruzi recombinant protein
Année de publication
1996
Type de document
Article
Auteurs
Guevara A.G., Taibi A., Billaut-Mulot O., Lemesre Jean-Loup, Ouaissi A.
Source
Memorias do Instituto Oswaldo Cruz, 1996, 91 (no spécial), p. 255 ISSN 0074-0276
In previous studies, we and others have shown utility of a 24-kDa Trypanosoma cruzi recombinant antigen (rTc24) for serological diagnosis of Chagas' disease. Also, this molecule has been proved useful to evaluate cure of chagasic patients who submit to specific treatment. However, in all the studies done so far, the 24-kDa protein was used as a fusion with a Gluthatione-S-transferase (GST) of Schistosoma japonicum, therefore, parallel assays to determine the anti-GST responses of all sera were required to deduce the GST noise in serological tests. Here, we show the subcloning by polymerase chain reaction of the cDNA encoding the T. cruzi 24-kDa antigen in a vector system (pQE) allowing us to obtain Tc24 recombinant protein as a single molecule. The highly reactivity of chagasic sera from Colombia, Ecuador, Brazil and Bolivia in ELISA against the recombinant antigen is confirmed. However, sera from patients infected with African trypanosomes recognize rTc24 in ELISA and blot. The relevance of these findings in the context of Chagas' disease diagnosis and/or the relationship with African trypanosomes is analyzed. (Résumé d'auteur)
Plan de classement
Epidémiologie des trypanosomiases [052GLOTRY02]
Descripteurs
ANTIGENE ; CLONE ; TRYPANOSOMIASE HUMAINE ; PCR.REACTION DE POLYMERISATION EN CHAINE ; PROTEINE RECOMBINANTE
Localisation
Fonds IRD [F B010007851]
Identifiant IRD
fdi:010007851
Contact