@article{fdi:010007849,
  title = {{M}ultiple primer {RAPD} analysis for studying genetic diversity and molecular taxonomy of {L}eishmania spp},
  author = {{B}anuls, {A}nne-{L}aure and {S}idib{\'e}, {I}ssa and {B}risse, {S}ylvain and {A}revalo, {J}. and {L}e {R}ay, {D}. and {T}ibayrenc, {M}ichel},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n order to study the genetic diversity of the #{L}eishmania$ genus, {R}andom {A}mplification of {P}olymorphic {DNA} ({RAPD}) was performed on a set of 18 #{L}eishmania$ stocks representative of the main species of the genus. {W}e tested on these sample one hundred decamer primers in parallel with samples of other microorganisms (#{T}. cruzi$, #{T}. congolense$, #{C}andida albicans$, #{M}. tuberculosis$). {S}everal lines of results have been reached. {T}wenty three primers gave easily scoreable multiband profiles. {T}he combined use of a fair set of these primers will make it possible to perform both, highly discriminative strain identification and population genetic analyses. {S}ome of them showed a synapomorphic specificity, that is to say : they can specifically identify given phylogenetic subdivisions, either at a subspecific or at a specific level. {F}or example, one primer showed a profile specific of #{L}eishmania peruviana$. {O}ther primers revealed different profiles between #{L}. guyanensis$, #{L}. braziliensis$, #{L}. lainsoni$ and #{L}. chagasi$. {L}astly, some primers appeared to be linked with specific virulence patterns. {S}ome of them, for example, seemed to be able to distinguish the stocks isolated from mucocutaneous forms from the stocks isolated from cutaneous forms for #{L}. braziliensis$. {T}he great interest of the {RAPD} method in comparison with isoenzyme analysis is to allow the purification and sequence characterisation of those fragments which are of a specific interest, for designing probes and {PCR} diagnoses. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{LEISHMANIOSE} ; {AGENT} {PATHOGENE} ; {POLYMORPHISME} {GENETIQUE} ; {TAXONOMIE} ; {ISOENZYME} ; {ETUDE} {COMPARATIVE} ; {TAXONOMIE} {BIOCHIMIQUE} ; {RAPD}.{RANDOM} {AMPLIFIED} {POLYMORPHIC} {DNA}},
  booktitle = {},
  journal = {{M}emorias do {I}nstituto {O}swaldo {C}ruz},
  volume = {91},
  numero = {no sp{\'e}cial},
  pages = {184},
  ISSN = {0074-0276},
  year = {1996},
  URL = {https://www.documentation.ird.fr/hor/fdi:010007849},
}