Banuls Anne-Laure, Sidibé Issa, Brisse Sylvain, Arevalo J., Le Ray D., Tibayrenc Michel. (1996). Multiple primer RAPD analysis for studying genetic diversity and molecular taxonomy of Leishmania spp. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 184. ISSN 0074-0276.
Titre du document
Multiple primer RAPD analysis for studying genetic diversity and molecular taxonomy of Leishmania spp
Année de publication
1996
Type de document
Article
Auteurs
Banuls Anne-Laure, Sidibé Issa, Brisse Sylvain, Arevalo J., Le Ray D., Tibayrenc Michel
Source
Memorias do Instituto Oswaldo Cruz, 1996,
91 (no spécial), p. 184 ISSN 0074-0276
In order to study the genetic diversity of the Leishmania genus, Random Amplification of Polymorphic DNA (RAPD) was performed on a set of 18 Leishmania stocks representative of the main species of the genus. We tested on these sample one hundred decamer primers in parallel with samples of other microorganisms (T. cruzi, T. congolense, Candida albicans, M. tuberculosis). Several lines of results have been reached. Twenty three primers gave easily scoreable multiband profiles. The combined use of a fair set of these primers will make it possible to perform both, highly discriminative strain identification and population genetic analyses. Some of them showed a synapomorphic specificity, that is to say : they can specifically identify given phylogenetic subdivisions, either at a subspecific or at a specific level. For example, one primer showed a profile specific of Leishmania peruviana. Other primers revealed different profiles between L. guyanensis, L. braziliensis, L. lainsoni and L. chagasi. Lastly, some primers appeared to be linked with specific virulence patterns. Some of them, for example, seemed to be able to distinguish the stocks isolated from mucocutaneous forms from the stocks isolated from cutaneous forms for L. braziliensis. The great interest of the RAPD method in comparison with isoenzyme analysis is to allow the purification and sequence characterisation of those fragments which are of a specific interest, for designing probes and PCR diagnoses. (Résumé d'auteur)
