Soares R.P.P., Dujardin Jean-Pierre, Romanha A.J., Santoro M.M., Schofield C.J., Diotaiuti L. (1996). The effect of laboratory maintenance of Panstrongylus megistus on its flight activity. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 142. ISSN 0074-0276.
Titre du document
The effect of laboratory maintenance of Panstrongylus megistus on its flight activity
Année de publication
1996
Auteurs
Soares R.P.P., Dujardin Jean-Pierre, Romanha A.J., Santoro M.M., Schofield C.J., Diotaiuti L.
Source
Memorias do Instituto Oswaldo Cruz, 1996,
91 (no spécial), p. 142 ISSN 0074-0276
In this study we compared two lines of P. megistus which differed only by its laboratory maintenance time. PM1 (more than five generations in insectarium) and PM2 (one or two generations under laboratory conditions). The parameters studied were temperature (tp), humidity (hm), the weight/length ratios (nutritional status, w/l), isoenzyme electrophoresis at 7 locus (ME, GPI, LDH, ICD and alfa-GPD), as well as enzymatic activity of alfa-glycerophosphate dehydrogenase (alfa-GPD ac.), a crucial flight activity related enzyme. Insects were processed according to SCHOFIELD (1980) and observed througth a period of 30 days. The temperature and humidity were measured daily at the safe time, and the insects that had flighted, "good" flyers (gf), were also measured for the determination of the nutritional status. Muscle extraction was made for isoenzyme and alfa-GPD enzymatic studies. At the end of this period the same number of insects that did not fly, "bad" flyers (bf), were used as control and submitted to the same analysis. No isoenzyme difference was observed between gf and bf for the two populations. Main external factors influencing flight activity were w/l (PM1 P=0.0027; PM2 P=0.0209) and tp (PM1 P=0.0814; PM2 P=0.0118). However, PM1 and PM2 strongly differed with respect to alfa-GPD activity : it was the main difference found between PM1-gf and PM1-bf (P=0.0001), whereas it did not present any evidence for heterogeneity in PM2 (P=0.5995). We used two multivariate approaches to verify the differences between gf and bf (logistic regression and canonical variate analysis, CVA). Logistic regression was able to separate "good" from "bad" flyers of the old established laboratory strain (PM1) using as the unique parameter value the alfa-GPD activity (87 to 93% of correct attribution), while it reached lower scores (78 to 86%) in PM2, needing the combination of two or more parameters excluding alfa-GPD activity. (Résumé d'auteur)
Plan de classement
Autres insectes [052AUTINS]
Descripteurs
BIOLOGIE ; ETHOLOGIE ; ELEVAGE DE LABORATOIRE ; TEMPERATURE ; HUMIDITE DE L'AIR ; ETAT NUTRITIONNEL ; ISOENZYME ; ENZYME
Localisation
Fonds IRD [F B010007845]
Identifiant IRD
fdi:010007845
