Brenière Simone Frédérique, Bosseno Marie-France, Noireau François. (1996). Circulation and behaviour of two major clones of Trypanosoma cruzi in Bolivian cycles. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 42-43. ISSN 0074-0276.
Titre du document
Circulation and behaviour of two major clones of Trypanosoma cruzi in Bolivian cycles
Année de publication
1996
Type de document
Article
Auteurs
Brenière Simone Frédérique, Bosseno Marie-France, Noireau François
Source
Memorias do Instituto Oswaldo Cruz, 1996,
91 (no spécial), p. 42-43 ISSN 0074-0276
On the basis of isoenzyme studies and population genetic interpretation, we have previously designed a molecular identification tool of two major clones of Trypanosoma cruzi based on both kDNA PCR amplification and southern hybridization using clone specific probes. These tools allow the direct identification of two genetic sub-groups of clones, genetically unrelated in biological samples. In the main vector of Chagas' disease in Bolivia (Triatoma infestans) we show that these two groups of clones are wide spread over large geographic distances and infect 92% of triatomes. We also observe high rates of mixed infections, ranging from 7.7% to 85.7% according to the localities. In a high endemic area, we show a significant difference of clone distribution between vectors and chagasic children. We have some arguments to think that both groups of clones are not selected during the transmission to man but that later, the control of parasitemia would be more effective over one of the two groups of clone. The second potential vector in Bolivia is Triatoma sordida in some areas. Both groups of clones are occasionally found in this vector, but the large majority of clones are genetically unrelated to them. These data support, a sylvatic origin of both groups of clones further catch by T. infestans domestic vector, the scarity of present contacts between both cycles sylvatic and domestic. (Résumé d'auteur)
Plan de classement
Epidémiologie des trypanosomiases [052GLOTRY02]
Descripteurs
MALADIE DE CHAGAS ; EPIDEMIOLOGIE ; VECTEUR ; TRANSMISSION ; IDENTIFICATION ; POLYMORPHISME GENETIQUE ; CLONE ; PCR.REACTION DE POLYMERISATION EN CHAINE
