@article{fdi:010007740,
  title = {{I}solation and expression in transgenic tobacco and rice plants of the cassava vein mosaic virus ({CVMV}) promoter},
  author = {{V}erdaguer, {B}. and {K}ochko, {A}lexandre de and {B}eachy, {R}.{N}. and {F}auquet, {C}laude},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he cassava vein mosaic virus ({CVMV}) is a double stranded {DNA} virus which infects cassava plants (#{M}anihot esculenta$ {C}rantz) and has been characterized as a plant pararetrovirus belonging to the caulimovirus subgroup. {T}wo {DNA} fragments, {CVP}1 of 388 nucleotides from position -386 to +20 and {CVP}2 of 511 nucleotides from position -443 to +72, were isolated from the viral genome and fused to the uid{A} reporter gene to test promoter expression. {T}he transcription start site of the viral promoter was determined using {RNA} isolated from transgenic plants containing the {CVMV} promoter:uid{A} fusion gene. {B}oth promoter fragments were able to cause high levels of gene expression in protoplasts isolated from cassava and tobacco cell suspensions. {T}he expression pattern of the {CVMV} promoters was analyzed in transgenic tobacco and rice plants, and revealed that the {GUS} staining pattern was similar for each construct and in both plants. {T}he two promoter fragments were active in all plant organs tested and in a variety of cell types, suggesting a near constitutive pattern of expression. {I}n both tobacco and rice plants, {GUS} activity was highest in vascular elements, in leaf mesophyll cells, and in root tips. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{MALADIE} {DES} {PLANTES} ; {VIRUS} ; {BIOLOGIE} {MOLECULAIRE} ; {GENIE} {GENETIQUE} ; {GENE} ; {PROTOPLASTE} ; {PLANTE} {TRANSGENIQUE}},
  booktitle = {},
  journal = {{P}lant {M}olecular {B}iology},
  volume = {31},
  numero = {},
  pages = {1129--1139},
  ISSN = {0167-4412},
  year = {1996},
  DOI = {10.1007/{BF}00040830},
  URL = {https://www.documentation.ird.fr/hor/fdi:010007740},
}