%0 Conference Proceedings
%9 ACTI : Communications avec actes dans un congrès international
%A Garraud, O.
%A Diouf, A.
%A Longacre, S.
%A Kaslow, D.C.
%A Holder, A.A.
%A Dieye, A.
%A Tall, A.
%A Roussihon, C.
%A Trape, Jean-François
%A Rogier, C.
%A Perraut, R.
%A Mercereau-Puijalon, O.
%T In vitro responses of senegalese adults PBMC to recombinant MSP1 19 antigens depends on expression systems and/or Plasmodium falciparum exposure
%S Program and abstract of the 45th annual meeting of the American Society of Tropical Medicine and Hygiene
%D 1996
%L fdi:010007002
%G ENG
%J American Journal of Tropical Medicine and Hygiene
%@ 0002-9637
%K PALUDISME ; VECTEUR ; INFESTATION ; IMMUNOLOGIE ; ANTIGENE ; AGENT PATHOGENE ; TRANSMISSION
%K REPONSE IMMUNE ; ANTIGENE RECOMBINANT ; ANTIGENE MSP1
%K SENEGAL
%N 2
%P 134
%U https://www.documentation.ird.fr/hor/fdi:010007002
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010007002.pdf
%V 55
%W Horizon (IRD)
%X To investigate the potential influence of antigen (Ag) presentation on the immune response to MSP1 19, the 19 kDa C-terminal domain of the #Plasmodium falciparum$ MSP1 Ag, 3 recombinant MSP 19 Ags expressed in baculovirus, #E. coli$ and #Saccharomyces cerevisiae$ were used to stimulate in vitro peripheral blood mononuclear cells (PBMCS) from #P. falciparum$ immune adults with no recent history of clinical malaria. Donors were recruited in 2 senegalese villages (Dielmo and Ndiop), where malaria transmission is perennial and seasonal, respectively. Each PBMC preparation was stimulated with a range of concentrations of the 3 recombinant Ags (cleaved from carrier molecules when appropriate). Most subjects responded to at least 1 recombinant Ag (SI > 3 in 8/10 at Dielmo and 14/15 at Ndiop). The dose-dependent response to #Saccharomyces cerevisiae$ MSP1 19 was similar in both villages. In contrast, there was a 10-fold difference in the optimal concentration of the #E. coli$ product between the two villages. Both the optimal dose and the concentration-dependence of the simulation by the baculovirus recombinant protein differed in each setting. These studies show that the 3 recombinant antigens differ in their capacity to stimulate lymphocyte proliferation, suggesting that protein structure (folding and/or glycosylation) affecting Ag processing, is critical. Futhermore, these data suggest that the malaria transmission pattern influences the immune response to the various MSP1 19 recombinants. (Résumé d'auteur)
%B Annual Meeting of the American Society of Tropical Medicine and Hygiene
%8 1996/12/1-5
%$ 052ANOPAL03