Mahmoud A., Thouvenel Jean-Claude, Abol-Ela S.E., Sewify G.H., Ammar E.D. (1996). Detection of maize yellow stripe tenui-like virus by ELISA and dot- blot tests in host plants and leafhopper vector in Egypt. Phytopathologia Mediterranea, 35 (1), p. 19-23. ISSN 0031-9465.
Titre du document
Detection of maize yellow stripe tenui-like virus by ELISA and dot- blot tests in host plants and leafhopper vector in Egypt
Année de publication
1996
Type de document
Article
Auteurs
Mahmoud A., Thouvenel Jean-Claude, Abol-Ela S.E., Sewify G.H., Ammar E.D.
Source
Phytopathologia Mediterranea, 1996,
35 (1), p. 19-23 ISSN 0031-9465
Antisera to the nucleoprotein of maize yellow stripe tenui-like virus (MYSV) were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oats, and the graminaceous weeds Bromus wildenowii, Cenchrus biflorus, Dichanthium annulatum, Digitaria sanguinalis, Echinochloa colonum, Setaria verticillata and S. viridis. In maize leaves, differences in virus titer appeared to be correlated with leaf age and with MYSV symptom-types. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers (Cicadulina chinai). (Résumé d'auteur)
Plan de classement
Symptomatologie, diagnostic des agents pathogènes [076MALPLA03]
