@article{fdi:010006167, title = {{S}ingle-standed {DNA} conformation polymorphism at the {R}dl locus in {H}ypothenemus hampei ({C}oleoptera : {S}colytidae)}, author = {{B}orsa, {P}hilippe and {C}oustau, {C}.}, editor = {}, language = {{ENG}}, abstract = {{T}he homologue of the resistance to dieldrin gene ({R}dl) in #{D}rosophila melanogaster$ was cloned and sequenced in the scolytid beetle #{H}ypothenemus hampei$, a coffee pest resistant to cyclodiene insecticides in {N}ew {C}aledonia. {T}he amino acid sequence of the {R}dl exon no. 7 protein product in #{H}. hampei$ was identical to that in #{D}. melanogaster$ and showed the same amino acid change as that characterizing susceptible vs. resistant #{D}. melanogaster$. {S}amples from natural #{H}. hampei$ populations (from {A}sia, the {P}acific {I}slands, {A}frica and {C}entral {A}merica), from reference susceptible ({S}) and resistant ({R}) laboratory strains, and from their hybrid progenies, were analysed at the {R}dl locus using single-stranded {DNA} conformation polymorphism on polymerase chain reaction products. {T}he susceptible allele was the only allele present in all samples from natural populations except in the only resistant population known to date ({P}onerihouen, {N}ew {C}aledonia). {F}emales and some males obtained as {F}1 from {R} {X} {S} crosses were heterozygous at the {R}dl locus, confirming that this local mate competing species is diplo-diploid. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{ENTOMOLOGIE} {APPLIQUEE} ; {INSECTE} {NUISIBLE} ; {CAFE} ; {INSECTICIDE} {CHIMIQUE} ; {SENSIBILITE} {RESISTANCE} ; {GENETIQUE} ; {ADN} ; {POLYMORPHISME}}, booktitle = {}, journal = {{H}eredity}, volume = {76}, numero = {}, pages = {124--129}, ISSN = {0018-067{X}}, year = {1996}, DOI = {10.1038/hdy.1996.19}, URL = {https://www.documentation.ird.fr/hor/fdi:010006167}, }