@article{fdi:010005866, title = {{D}escription of {T}hermoanaerobacter brockii subsp. lactiethylicus subsp. nov. isolated from a deep subsurface french oil well : a proposal to reclassify {T}hermoanaerobacter finnii as {T}hermoanaerobacter brockii subsp. finnii comb. nov. and an emended description of {T}hermoanaerobacter brockii}, author = {{C}ayol, {J}ean-{L}uc and {O}llivier, {B}ernard and {P}atel, {B}.{K}.{C}. and {R}avot, {G}. and {M}agot, {M}. and {A}geron, {E}. and {G}rimont, {P}.{A}.{D}. and {G}arcia, {J}ean-{L}ouis}, editor = {}, language = {{ENG}}, abstract = {{A} strictly anaerobic, thermophilic, gram-positive, spore-forming eubacterium designated strain {SEBR} 5268 was isolated from an oil field at a depth of 2,100 m, where the temperature was 92°{C}. {T}he cells of this organism were gram-positive, straight, motile rods (0,5 by 2 to 3 micrometers) with peritrichous flagella. {T}he cells occurred singly or in pairs during the logarithmic growth phase, but were pleomorphic and filamentous (length, 15 micrometers) in old cultures. {G}rowth occurred at temperatures of 40 to 75°{C}, and optimum growth occurred at temperatures between 55 and 60°{C}. {T}he fermentable substrates included glucose, fructose, galactose, mannose, cellobiose, maltose, sucrose, lactose, {D}-xylose, {D}-ribose, mannitol, pyruvate, and starch. {T}he products of fermentation of glucose were lactate, acetate, ethanol, {H}2, and {CO}2. {T}he {DNA} base composition was 35 mol% {G}+{C}. {T}he results of 16{S} r{RNA} sequence comparisons indicated that strain {SEBR} 5268 was closely related to #{T}hermoanaerobacter brockii$ and #{T}hermoanaerobacter finnii$, and these three organims exhibited levels of ribosomal {DNA} sequence homology of 98 to 99 %. {T}he results of {DNA}-{DNA} hybridization studies performed with the three organisms confirmed this close affiliation, and as base pairing values of > 70 % were obtained, these organisms belong to the same species. {T}herefore, we propose that #{T}. finnii$ should be reclassified as a subspecies of #{T}. brockii$, #{T}hermoanaerobacter brockii$ subsp. #finnii$ comb. nov. {T}his automatically creates #{T}hermoanaerobacter brockii$ subsp. #brockii$. {W}e also propose that strain {SEBR} 5268 should be classified as a member of a new subspecies of #{T}. brockii$, #{T}hermoanaerobacter brockii$ subsp. #lactiethylicus$. {T}he latter differs from #{T}. brockii$ subsp. #brockii$ and #{T}. brockii$ subsp. #finnii$ by its 16{S} r{RNA} sequence, {DNA} sequence diversity, lower temperature optimum, {G}+{C} content, and carbohydrate utilization spectrum. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{FERMENTATION} ; {BACTERIOLOGIE} ; {BACTERIE} {THERMOPHILE} ; {METABOLISME} ; {TAXONOMIE} ; {ADN}}, booktitle = {}, journal = {{I}nternational {J}ournal of {S}ystematic {B}acteriology}, volume = {45}, numero = {4}, pages = {783--789}, ISSN = {0020-7713}, year = {1995}, URL = {https://www.documentation.ird.fr/hor/fdi:010005866}, }