Guillemaud T., Raymond M., Callot G., Cleyet-Marel J.C., Fernandez Diana. (1996). Variability of nuclear and mitochondrial ribosomal DNA of a truffle species (Tuber aestivum). Mycological Research, 100 (5), p. 547-550. ISSN 0953-7562.
Titre du document
Variability of nuclear and mitochondrial ribosomal DNA of a truffle species (Tuber aestivum)
Année de publication
1996
Type de document
Article
Auteurs
Guillemaud T., Raymond M., Callot G., Cleyet-Marel J.C., Fernandez Diana
Source
Mycological Research, 1996,
100 (5), p. 547-550 ISSN 0953-7562
The intraspecific genetic variability of Tuber aestivum was studied using molecular markers at various geographical scales. We used the polymerase chain reaction (PCR) coupled with restriction fragment length polymorphism (RFLP) analysis to examine the variation of the nuclear and mitochondrial ribosomal DNA (rDNA). RFLPs were found in the nuclear internal transcribed spacer (ITS) and three alleles were detected in the six populations analysed. No variability was found in mitochondrial rDNA. We found, in a very few cases, that truffles sharing different ITS genotypes could be present within a single symbiotic tree. (Résumé d'auteur)
Plan de classement
Biologie [020BIOL]
;
Champignons [074MICFLO03]
Descripteurs
CHAMPIGNON ; VARIABILITE GENETIQUE ; ADN ; NOYAU CELLULAIRE ; MITOCHONDRIE ; PCR.REACTION DE POLYMERISATION EN CHAINE ; RFLP.RESTRICTION FRAGMENT LENGTH POLYMORPHISM
