%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Nicolas, L. %A Luquiaud, P. %A Lardeux, Frédéric %A Mercer, D.R. %T A polymerase chain reaction assay to determine infection of Aedes polynesiensis by Wuchereria bancrofti %D 1996 %L fdi:010005228 %G ENG %J Transactions of the Royal Society of Tropical Medicine and Hygiene %@ 0035-9203 %K FILARIOSE LYMPHATIQUE ; VECTEUR ; DEPISTAGE ; TEST ; AGENT PATHOGENE %K PCR.REACTION DE POLYMERISATION EN CHAINE %N 2 %P 136-139 %R 10.1016/S0035-9203(96)90113-3 %U https://www.documentation.ird.fr/hor/fdi:010005228 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_43-44/010005228.pdf %V 90 %W Horizon (IRD) %X The sensitivity of a previously described polymerase chain reaction (PCR) assay was improved to detect a single mosquito, infected by as few as 1-2 microfilariae of #Wuchereria bancrofti$, among 20-50 uninfected mosquitoes. Wild-caught #Aedes polynesiensis$ were used to compare assessment of infection by dissection of individuals with the PCR assay of pools of mosquitoes. The PCR assay was at least as sensitive as dissection for detection of mosquitoes infected with #W. bancrofti$. (Résumé d'auteur) %$ 052MALTRA05