@article{PAR00015359,
  title = {{A}cquisition of high diversity of bacteria during the {H}ajj pilgrimage, including {A}cinetobacter baumannii with bla({OXA}-72) and {E}scherichia coli with bla({NDM}-5) carbapenemase genes},
  author = {{L}eangapichart, {T}. and {G}autret, {P}. and {G}riffiths, {K}. and {B}elhouchat, {K}. and {M}emish, {Z}. and {R}aoult, {D}idier and {R}olain, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}ilgrims returning from the {H}ajj (pilgrimage to {M}ecca) can be carriers of multidrug-resistant bacteria ({MDR}). {P}haryngeal and rectal swab samples were collected from 98 pilgrims before and after they traveled to the {H}ajj in 2014 to investigate the acquisition of {MDR} bacteria. {T}he bacterial diversity in pharyngeal swab samples was assessed by culture with selective media. {T}here was a significantly higher diversity of bacteria in samples collected after the return from the {H}ajj than in those collected before ({P} = 0.0008). {S}urprisingly, {A}cinetobacter baumannii strains were isolated from 16 pharyngeal swab samples (1 sample taken during the {H}ajj and 15 samples taken upon return) and 26 post-{H}ajj rectal swab samples, while none were isolated from samples taken before the {H}ajj. {T}esting of all samples by real-time {PCR} targeting bla({OXA}-51) gave positive results for only 1% of samples taken during the {H}ajj, 21/90 (23.3%) pharyngeal swab samples taken post-{H}ajj, and 35/90 (38.9%) rectal swab samples taken post-{H}ajj. {O}ne strain of {A}. baumannii isolated from the pharynx was resistant to imipenem and harbored a bla({OXA}-72) carbapenemase gene. {M}ultilocus sequence typing analysis of 43 {A}. baumannii isolates revealed a huge diversity of 35 sequence types ({ST}s), among which 18 were novel {ST}s reported for the first time in this study. {M}oreover, we also found one {E}scherichia coli isolate, collected from a rectal swab sample from a pilgrim taken after the {H}ajj, which harbored bla({NDM}-5), bla({CTX}-{M}-15), bla({TEM}-1), and aad{A}2 ({ST}2659 and {ST}181). {I}n conclusion, pilgrims are at a potential risk of acquiring and transmitting {MDR} {A}cinetobacter spp. and carbapenemase-producing {G}ram-negative bacteria during the {H}ajj season.},
  keywords = {{FRANCE} ; {ARABIE} {SAOUDITE}},
  booktitle = {},
  journal = {{A}ntimicrobial {A}gents and {C}hemotherapy},
  volume = {60},
  numero = {10},
  pages = {5942--5948},
  ISSN = {0066-4804},
  year = {2016},
  DOI = {10.1128/aac.00669-16},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00015359},
}