%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gouriet, F.
%A Ghiab, F.
%A Couderc, C.
%A Bittar, F.
%A Dupont, H. T.
%A Flaudrops, C.
%A Casalta, J. P.
%A Sambe-Ba, B.
%A Fall, B.
%A Raoult, Didier
%A Fenollar, F.
%T Evaluation of a new extraction protocol for yeast identification by mass spectrometry
%D 2016
%L PAR00015079
%G ENG
%J Journal of Microbiological Methods
%@ 0167-7012
%K Yeast ; Mass spectrometry ; Identification
%M ISI:000383941500010
%P 61-65
%R 10.1016/j.mimet.2016.08.001
%U https://www.documentation.ird.fr/hor/PAR00015079
%V 129
%W Horizon (IRD)
%X In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.
%$ 084 ; 020