Gouriet F., Ghiab F., Couderc C., Bittar F., Dupont H. T., Flaudrops C., Casalta J. P., Sambe-Ba B., Fall B., Raoult Didier, Fenollar F. (2016). Evaluation of a new extraction protocol for yeast identification by mass spectrometry. Journal of Microbiological Methods, 129, p. 61-65. ISSN 0167-7012.
Titre du document
Evaluation of a new extraction protocol for yeast identification by mass spectrometry
Gouriet F., Ghiab F., Couderc C., Bittar F., Dupont H. T., Flaudrops C., Casalta J. P., Sambe-Ba B., Fall B., Raoult Didier, Fenollar F.
Source
Journal of Microbiological Methods, 2016,
129, p. 61-65 ISSN 0167-7012
In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Biotechnologies [084]
