%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Brioude, G.
%A Bregeon, F.
%A Trousse, D.
%A Flaudrops, C.
%A Secq, V.
%A De Dominicis, F.
%A Chabrieres, E.
%A D'Journo, X. B.
%A Raoult, Didier
%A Thomas, P. A.
%T Rapid diagnosis of lung tumors, a feasability study using Maldi-Tof mass spectrometry
%D 2016
%L PAR00014697
%G ENG
%J Plos One
%@ 1932-6203
%M ISI:000376882500020
%N 5
%P e0155449
%R 10.1371/journal.pone.0155449
%U https://www.documentation.ird.fr/hor/PAR00014697
%V 11
%W Horizon (IRD)
%X Objective Despite recent advances in imaging and core or endoscopic biopsies, a percentage of patients have a major lung resection without diagnosis. We aimed to assess the feasibility of a rapid tissue preparation/analysis to discriminate cancerous from non-cancerous lung tissue. Methods Fresh sample preparations were analyzed with the Microflex LTTM MALDI-TOF analyzer. Each main reference spectra (MSP) was consecutively included in a database. After definitive pathological diagnosis, each MSP was labeled as either cancerous or non-cancerous (normal, inflammatory, infectious nodules). A strategy was constructed based on the number of concordant responses of a mass spectrometry scoring algorithm. A 3-step evaluation included an internal and blind validation of a preliminary database (n = 182 reference spectra from the 100 first patients), followed by validation on a whole cohort database (n = 300 reference spectra from 159 patients). Diagnostic performance indicators were calculated. Results 127 cancerous and 173 non-cancerous samples (144 peripheral biopsies and 29 inflammatory or infectious lesions) were processed within 30 minutes after biopsy sampling. At the most discriminatory level, the samples were correctly classified with a sensitivity, specificity and global accuracy of 92.1%, 97.1% and 95%, respectively. Conclusions The feasibility of rapid MALDI-TOF analysis, coupled with a very simple lung preparation procedure, appears promising and should be tested in several surgical settings where rapid on-site evaluation of abnormal tissue is required. In the operating room, it appears promising in case of tumors with an uncertain preoperative diagnosis and should be tested as a complementary approach to frozen-biopsy analysis.
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