@article{PAR00014697,
  title = {{R}apid diagnosis of lung tumors, a feasability study using {M}aldi-{T}of mass spectrometry},
  author = {{B}rioude, {G}. and {B}regeon, {F}. and {T}rousse, {D}. and {F}laudrops, {C}. and {S}ecq, {V}. and {D}e {D}ominicis, {F}. and {C}habrieres, {E}. and {D}'{J}ourno, {X}. {B}. and {R}aoult, {D}idier and {T}homas, {P}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective {D}espite recent advances in imaging and core or endoscopic biopsies, a percentage of patients have a major lung resection without diagnosis. {W}e aimed to assess the feasibility of a rapid tissue preparation/analysis to discriminate cancerous from non-cancerous lung tissue. {M}ethods {F}resh sample preparations were analyzed with the {M}icroflex {LTTM} {MALDI}-{TOF} analyzer. {E}ach main reference spectra ({MSP}) was consecutively included in a database. {A}fter definitive pathological diagnosis, each {MSP} was labeled as either cancerous or non-cancerous (normal, inflammatory, infectious nodules). {A} strategy was constructed based on the number of concordant responses of a mass spectrometry scoring algorithm. {A} 3-step evaluation included an internal and blind validation of a preliminary database (n = 182 reference spectra from the 100 first patients), followed by validation on a whole cohort database (n = 300 reference spectra from 159 patients). {D}iagnostic performance indicators were calculated. {R}esults 127 cancerous and 173 non-cancerous samples (144 peripheral biopsies and 29 inflammatory or infectious lesions) were processed within 30 minutes after biopsy sampling. {A}t the most discriminatory level, the samples were correctly classified with a sensitivity, specificity and global accuracy of 92.1%, 97.1% and 95%, respectively. {C}onclusions {T}he feasibility of rapid {MALDI}-{TOF} analysis, coupled with a very simple lung preparation procedure, appears promising and should be tested in several surgical settings where rapid on-site evaluation of abnormal tissue is required. {I}n the operating room, it appears promising in case of tumors with an uncertain preoperative diagnosis and should be tested as a complementary approach to frozen-biopsy analysis.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {11},
  numero = {5},
  pages = {e0155449},
  ISSN = {1932-6203},
  year = {2016},
  DOI = {10.1371/journal.pone.0155449},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014697},
}