Brioude G., Bregeon F., Trousse D., Flaudrops C., Secq V., De Dominicis F., Chabrieres E., D'Journo X. B., Raoult Didier, Thomas P. A. (2016). Rapid diagnosis of lung tumors, a feasability study using Maldi-Tof mass spectrometry. Plos One, 11 (5), p. e0155449. ISSN 1932-6203.
Titre du document
Rapid diagnosis of lung tumors, a feasability study using Maldi-Tof mass spectrometry
Année de publication
2016
Auteurs
Brioude G., Bregeon F., Trousse D., Flaudrops C., Secq V., De Dominicis F., Chabrieres E., D'Journo X. B., Raoult Didier, Thomas P. A.
Source
Plos One, 2016,
11 (5), p. e0155449 ISSN 1932-6203
Objective Despite recent advances in imaging and core or endoscopic biopsies, a percentage of patients have a major lung resection without diagnosis. We aimed to assess the feasibility of a rapid tissue preparation/analysis to discriminate cancerous from non-cancerous lung tissue. Methods Fresh sample preparations were analyzed with the Microflex LTTM MALDI-TOF analyzer. Each main reference spectra (MSP) was consecutively included in a database. After definitive pathological diagnosis, each MSP was labeled as either cancerous or non-cancerous (normal, inflammatory, infectious nodules). A strategy was constructed based on the number of concordant responses of a mass spectrometry scoring algorithm. A 3-step evaluation included an internal and blind validation of a preliminary database (n = 182 reference spectra from the 100 first patients), followed by validation on a whole cohort database (n = 300 reference spectra from 159 patients). Diagnostic performance indicators were calculated. Results 127 cancerous and 173 non-cancerous samples (144 peripheral biopsies and 29 inflammatory or infectious lesions) were processed within 30 minutes after biopsy sampling. At the most discriminatory level, the samples were correctly classified with a sensitivity, specificity and global accuracy of 92.1%, 97.1% and 95%, respectively. Conclusions The feasibility of rapid MALDI-TOF analysis, coupled with a very simple lung preparation procedure, appears promising and should be tested in several surgical settings where rapid on-site evaluation of abnormal tissue is required. In the operating room, it appears promising in case of tumors with an uncertain preoperative diagnosis and should be tested as a complementary approach to frozen-biopsy analysis.
Plan de classement
Santé : généralités [050]
Identifiant IRD
PAR00014697
