%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Dubourg, G.
%A Lagier, J. C.
%A Robert, C.
%A Armstrong, N.
%A Couderc, C.
%A Fournier, P. E.
%A Raoult, Didier
%T Risungbinella massiliensis sp. nov., a new member of Thermoactinomycetaceae isolated from human gut
%D 2016
%L PAR00014696
%G ENG
%J Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
%@ 0003-6072
%K Genome ; Culturomics ; Taxonogenomics
%M ISI:000376012300003
%N 6
%P 773-784
%R 10.1007/s10482-016-0677-6
%U https://www.documentation.ird.fr/hor/PAR00014696
%V 109
%W Horizon (IRD)
%X A novel filamentous bacterium, designated GD1(T), was isolated from the gut microbiota of a 38-year-old male who suffered from a Coxiella burnetii vascular for which he received multiple a broad-spectrum antibiotic cocktail at the time of the stool collection. The strain was isolated as a part of culturomics study by cultivation on 5 % sheep blood agar in aerobic condition at 28 A degrees C, after 14 days of incubation. Strain GD1(T) shows 16S rRNA gene sequence similarities of 98.01 % to the type strain of Risungbinella pyongyangensis. We describe here the features of this bacterium, together with the complete genome sequence and annotation. The G+C content of the genomic DNA was determined to be 40.1 mol %. The major fatty acids of strain GD1(T) were identified as iso-C15:0, iso-C17:0, anteiso-C15:0, iso-C14:0 and C16:0. The 3,440,191 bp long genome contains 3540 protein-coding and 67 RNA genes, including three rRNA genes. Strain GD1(T) (= DSM 46691 = CSUR P1082) sp. nov. is here classified as the type strain of a new species, Risungbinella massiliensis, within the family Thermoactinomycetaceae. To date, strain GD1(T) is the first member of the family Thermoactinomycetaceae isolated from human gut and the fourth from a human specimen.
%$ 054 ; 084