Dubourg G., Lagier J. C., Robert C., Armstrong N., Couderc C., Fournier P. E., Raoult Didier. (2016). Risungbinella massiliensis sp. nov., a new member of Thermoactinomycetaceae isolated from human gut. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 109 (6), p. 773-784. ISSN 0003-6072.
Titre du document
Risungbinella massiliensis sp. nov., a new member of Thermoactinomycetaceae isolated from human gut
Dubourg G., Lagier J. C., Robert C., Armstrong N., Couderc C., Fournier P. E., Raoult Didier
Source
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2016,
109 (6), p. 773-784 ISSN 0003-6072
A novel filamentous bacterium, designated GD1(T), was isolated from the gut microbiota of a 38-year-old male who suffered from a Coxiella burnetii vascular for which he received multiple a broad-spectrum antibiotic cocktail at the time of the stool collection. The strain was isolated as a part of culturomics study by cultivation on 5 % sheep blood agar in aerobic condition at 28 A degrees C, after 14 days of incubation. Strain GD1(T) shows 16S rRNA gene sequence similarities of 98.01 % to the type strain of Risungbinella pyongyangensis. We describe here the features of this bacterium, together with the complete genome sequence and annotation. The G+C content of the genomic DNA was determined to be 40.1 mol %. The major fatty acids of strain GD1(T) were identified as iso-C15:0, iso-C17:0, anteiso-C15:0, iso-C14:0 and C16:0. The 3,440,191 bp long genome contains 3540 protein-coding and 67 RNA genes, including three rRNA genes. Strain GD1(T) (= DSM 46691 = CSUR P1082) sp. nov. is here classified as the type strain of a new species, Risungbinella massiliensis, within the family Thermoactinomycetaceae. To date, strain GD1(T) is the first member of the family Thermoactinomycetaceae isolated from human gut and the fourth from a human specimen.
