Publications des scientifiques de l'IRD

Khelaifia S., Lagier J. C., Bibi F., Azhar E. I., Croce O., Padmanabhan R., Jiman-Fatani A. A., Yasir M., Robert C., Andrieu C., Fournier P. E., Raoult Didier. (2016). Microbial culturomics to map halophilic bacterium in human gut : genome sequence and description of Oceanobacillus jeddahense sp. nov.. Omics-a Journal of Integrative Biology, 20 (4), p. 248-258. ISSN 1536-2310.

Titre du document
Microbial culturomics to map halophilic bacterium in human gut : genome sequence and description of Oceanobacillus jeddahense sp. nov.
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000374760800007
Auteurs
Khelaifia S., Lagier J. C., Bibi F., Azhar E. I., Croce O., Padmanabhan R., Jiman-Fatani A. A., Yasir M., Robert C., Andrieu C., Fournier P. E., Raoult Didier
Source
Omics-a Journal of Integrative Biology, 2016, 20 (4), p. 248-258 ISSN 1536-2310
Culturomics is a new omics subspecialty to map the microbial diversity of human gut, coupled with a taxono-genomic strategy. We report here the description of a new bacterial species using microbial culturomics: strain S5(T), (=CSUR P1091=DSM 28586) isolated from a stool specimen of a 25-year-old obese patient from Saudi Arabia. The strain S5(T) was a Gram-positive, strictly aerobic rod, which was motile by a polar flagellum, spore-forming, and exhibited catalase and oxidase activities. It grows optimally at 37 degrees C, with a pH of 7.5 and 10% of NaCl. 16S rRNA gene-based identification revealed that strain S5(T) has 98.6% 16S rRNA sequence similarity with the reference O. oncorhynchi, phylogenetically the closest validated Oceanobacillus species. Here, we further describe the phenotypic characteristics of this organism and its complete genome sequence and annotation. The 5,388,285bp long genome exhibits a G+C content of 37.24% and contains 5109 protein-coding genes and 198 RNA genes. Based on the characteristics reported here, we propose classifying this novel bacterium as representative of a new species belonging to the genus Oceanobacillus, Oceanobacillus jeddahense sp. nov. In a broader context, it is noteworthy that halophilic bacteria have long been overlooked in the human gut, and their role in human health and disease has not yet been investigated. This study thus further underscores the usefulness of the culturomics approach exploring the bacterial diversity of the gut.
Plan de classement
Nutrition, alimentation [054] ; Biotechnologies [084]
Identifiant IRD
PAR00014695
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