@article{PAR00014694,
  title = {{A}erobic culture of methanogenic archaea without an external source of hydrogen},
  author = {{K}helaifia, {S}. and {L}agier, {J}. {C}. and {N}kamga, {V}. {D}. and {G}uilhot, {E}. and {D}rancourt, {M}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ulturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. {U}ntil now, these microorganisms have only been cultivated under strictly anaerobic conditions. {W}e previously developed a single versatile culture medium containing sugars and anti-oxydants for cultivating all human known methanogens. {P}erforming aerobic cultures in the presence of {B}acteroides thetaiotaomicron, which produces hydrogen, allows for cultivation of {M}ethanobrevibacter smithii which itself produces methane. {T}o obtain colonies, we cultivated {M}. smithii in an agar plate in the upper part of a double chamber flask with a liquid culture of {B}. thetaiotaomicron in the lower compartment. {W}e subsequently cultured four other methanogenic species for the first time and successfully isolated 13 strains of {M}. smithii and nine strains of {M}ethanobrevibacter oralis from 100 stools and 45 oral samples. {T}his procedure allows aerobic isolation and antibiotic susceptibility testing. {T}his changes the ability to routinely study methanogens, which have been neglected in clinical microbiology laboratories and may be useful for biogas production.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {35},
  numero = {6},
  pages = {985--991},
  ISSN = {0934-9723},
  year = {2016},
  DOI = {10.1007/s10096-016-2627-7},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014694},
}