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Khan I., Azhar E. I., Abbas A. T., Kumosani T., Barbour E. K., Raoult Didier, Yasir M. (2016). Metagenomic analysis of antibiotic-induced changes in gut microbiota in a pregnant rat model. Frontiers in Pharmacology, 7, p. art. 104. ISSN 1663-9812.

Titre du document
Metagenomic analysis of antibiotic-induced changes in gut microbiota in a pregnant rat model
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000374952100001
Auteurs
Khan I., Azhar E. I., Abbas A. T., Kumosani T., Barbour E. K., Raoult Didier, Yasir M.
Source
Frontiers in Pharmacology, 2016, 7, p. art. 104 ISSN 1663-9812
Food and Drug Administration (FDA, USA)-approved category B antibiotics are commonly prescribed to treat infections during pregnancy. The aim of this study was to investigate antibiotic-induced changes in gut microbiota (GM) that occur during pregnancy. The 16S rRNA amplicon deep-sequencing method was used to analyze the effect of category B antibiotics (azithromycin, amoxicillin and cefaclor) on GM during pregnancy using a rat model. The GM composition was substantially modulated by pregnancy and antibiotics administration. Firmicutes, Bacteroidetes, Proteobacteria,Chlamydiae, Actinobacteria, and Cyanobacteria were the dominant phyla. Antibiotic treatment during pregnancy increased the relative abundance of Proteobacteria and reduced Firmicutes. The genera Shigella, Streptococcus, Candidatus Arthromitus, and Helicobacter were significantly (p < 0.05) more abundant during pregnancy. Antibiotics significantly (p < 0.05) reduced the relative abundance of Lactobacillus but increased that of Enterobacter. There was a significant (p < 0.05) decrease in Lactobacillussp., Lactobacillus gallinarum and Lactobacillus crispatus during pregnancy. Antibiotictreatment reduced bacterial diversity; the lowest number of operational taxonomic units (OTUs) were detected in the cefaclor-treated groups. Antibiotics significantly (p < 0.05) promoted weight gain during pregnancy, and increased relative abundance of Shigella sonnei, Enterococcus hormaechei, and Acinetobacter sp. GM perturbations were accompanied by increases in Proteobacteria abundance and weight gain in pregnancy following antibiotic treatment.
Plan de classement
Santé : généralités [050] ; Nutrition, alimentation [054] ; Sciences du monde animal [080] ; Biotechnologies [084]
Identifiant IRD
PAR00014566
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