@article{PAR00014424,
  title = {{I}dentification of {A}lgerian field-caught phlebotomine sand fly vectors by maldi-tof ms},
  author = {{L}afri, {I}. and {A}lmeras, {L}. and {B}itam, {I}. and {C}aputo, {A}. and {Y}ssouf, {A}. and {F}orestier, {C}. {L}. and {I}zri, {A}. and {R}aoult, {D}idier and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {P}hlebotomine sand flies are known to transmit {L}eishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. {P}recise sand fly identification is essential to prevent phlebotomine-borne diseases. {O}ver the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}) has emerged as an accurate tool for arthropod identification. {T}he objective of the present study was to investigate the usefulness of {MALDI}-{TOF} {MS} as a tool for identifying field-caught phlebotomine. {M}ethodology/{P}rincipal {F}indings {S}and flies were captured in four sites in north {A}lgeria. {A} subset was morphologically and genetically identified. {S}ix species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. {T}he relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80 degrees {C}, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. {I}ntra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. {Q}uerying of the sand fly database using the {MS} spectra from the blind test groups revealed concordant results between morphological and {MALDI}-{TOF} {MS} identification. {H}owever, {MS} identification results were less efficient for specimens which were engorged or stored in alcohol. {I}dentification of 362 phlebotomine sand flies, captured at four {A}lgerian sites, by {MALDI}-{TOF} {MS}, revealed that the subgenus {L}arroussius was predominant at all the study sites, except for in {M}'sila where {P}.({P}hlebotomus) papatasi was the only sand fly species detected. {C}onclusion {T}he present study highlights the application of {MALDI}-{TOF} {MS} for monitoring sand fly fauna captured in the field. {T}he low cost, reliability and rapidity of {MALDI}-{TOF} {MS} analyses opens up new ways in the management of phlebotomine sand fly-borne diseases.},
  keywords = {{ALGERIE}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {10},
  numero = {1},
  pages = {e0004351},
  ISSN = {1935-2735},
  year = {2016},
  DOI = {10.1371/journal.pntd.0004351},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014424},
}