@article{PAR00014417,
  title = {{D}eglycosylation of {T}ropheryma whipplei biofilm and discrepancies between diagnostic results during {W}hipple's disease progression},
  author = {{A}udoly, {G}. and {F}enollar, {F}. and {L}agier, {J}. {C}. and {L}epidi, {H}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{W}hipple's disease is a systemic infectious disease associated with the bacterium {T}ropheryma whipplei. {N}umerous reports have presented puzzling discrepancies between diagnosis methods. {W}e addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and 1 lymph node biopsy from {W}hipple's patients. {W}e showed the presence of bacteria in both {CK}20(+) epithelial cells and {CD}68(+) macrophages. {B}acteria are found embedded in a biofilm hindering the detection of {T}. whipplei. {O}nly after treatment of biopsies by glycosidases, co-localization of {T}. whipplei {RNA}/{DNA} with bacterial proteins was restored. {M}oreover, using 13 bronchoalveolar lavages and 7 duodenal biopsies, we found that hydrolysis of the biofilm weakened the bacteria, facilitated bacterial {DNA} extraction and improved the sensitivity of q{PCR} detection by up to 1000x opening new perspectives for diagnostic and scientific approaches.},
  keywords = {{FRANCE}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {6},
  numero = {},
  pages = {art. 23883},
  ISSN = {2045-2322},
  year = {2016},
  DOI = {10.1038/srep23883},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014417},
}