@article{PAR00014300,
  title = {{I}dentification of blood meal sources in the main {A}frican malaria mosquito vector by {MALDI}-{TOF} {MS}},
  author = {{N}iare, {S}. and {B}erenger, {J}. {M}. and {D}ieme, {C}. and {D}oumbo, {O}. and {R}aoult, {D}idier and {P}arola, {P}. and {A}lmeras, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he identification of blood meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. {C}urrently, the identification of mosquito blood meal origins is based on time-consuming and costly techniques such as precipitin tests, {ELISA} and molecular tools. {A}lthough these tools have been validated to identify the blood meal and trophic preferences of female {A}nopheles mosquitoes, they present several limitations. {R}ecently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. {T}he aim of the present work was to test whether {MALDI}-{TOF} {MS} could also be applied to identification of blood meal sources from engorged mosquitoes. {M}ethods: {A}bdomen proteins extracted from {A}nopheles gambiae (stricto sensu, {S} molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for {MALDI}-{TOF} {MS}. {R}esults: {T}he comparison of mass spectrometry ({MS}) spectra from mosquito abdomens collected 1 h post-feeding, were able to discriminate blood meal origins. {M}oreover, using {A}edes albopictus specimens, abdominal protein {MS} spectra from engorged mosquitoes were found specific to host blood source and independent of the mosquito species. {A} sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post-feeding. {C}onclusions: {T}hese results indicate that {MALDI}-{TOF} {MS} could determine feeding patterns of freshly engorged mosquitoes up to 24 h post-blood meal. {T}he {MALDI}-{TOF} {MS} technique appears to be an efficient tool for large epidemiological surveillance of vector-borne diseases and outbreak source identification.},
  keywords = {{MALDI}-{TOF} mass spectrometry ; {B}lood meal source ; {A}nopheles ; {E}pidemiology ; {O}utbreak ; {S}urveillance ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {15},
  numero = {},
  pages = {art. 87},
  ISSN = {1475-2875},
  year = {2016},
  DOI = {10.1186/s12936-016-1152-6},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014300},
}