@article{PAR00014185,
  title = {{H}igh-throughput isolation of giant viruses in liquid medium using automated flow cytometry and fluorescence staining},
  author = {{K}halil, {J}. {Y}. {B}. and {R}obert, {S}. and {R}eteno, {D}. {G}. and {A}ndreani, {J}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he isolation of giant viruses using amoeba co-culture is tedious and fastidious. {R}ecently, the procedure was successfully associated with a method that detects amoebal lysis on agar plates. {H}owever, the procedure remains time-consuming and is limited to protozoa growing on agar. {W}e present here advances for the isolation of giant viruses. {A} high-throughput automated method based on flow cytometry and fluorescent staining was used to detect the presence of giant viruses in liquid medium. {D}evelopment was carried out with the {A}canthamoeba polyphaga strain widely used in past and current co-culture experiments. {T}he proof of concept was validated with virus suspensions: artificially contaminated samples but also environmental samples from which viruses were previously isolated. {A}fter validating the technique, and fortuitously isolating a new {M}imivirus, we automated the technique on 96-well plates and tested it on clinical and environmental samples using other protozoa. {T}his allowed us to detect more than 10 strains of previously known species of giant viruses and seven new strains of a new virus lineage. {T}his automated high-throughput method demonstrated significant time saving, and higher sensitivity than older techniques. {I}t thus creates the means to isolate giant viruses at high speed.},
  keywords = {giant viruses ; protozoa ; flow cytometry ; high-throughput ; automated system ; gating strategy ; fluorescence staining},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {7},
  numero = {},
  pages = {art. 26},
  ISSN = {1664-302{X}},
  year = {2016},
  DOI = {10.3389/fmicb.2016.00026},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00014185},
}