Teaniniuraitemoana V., Lepretre M., Levy P., Vanaa V., Parrad S., Gaertner-Mazouni Nabila, Gueguen Y., Huvet A., Le Moullac G. (2016). Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera. Journal of Experimental Zoology Part a-Ecological Genetics and Physiology, 325 (1), p. 13-24. ISSN 1932-5223.
Titre du document
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera
Année de publication
2016
Auteurs
Teaniniuraitemoana V., Lepretre M., Levy P., Vanaa V., Parrad S., Gaertner-Mazouni Nabila, Gueguen Y., Huvet A., Le Moullac G.
Source
Journal of Experimental Zoology Part a-Ecological Genetics and Physiology, 2016,
325 (1), p. 13-24 ISSN 1932-5223
The black-lip pearl oyster Pinctada margaritifera is a protandrous hermaphrodite species. Its economic value has led to the development of controlled hatchery reproduction techniques, although many aspects remain to be optimized. In order to understand reproductive mechanisms and their controlling factors, two independent experiments were designed to test hypotheses of gametogenesis and sex ratio control by environmental and hormonal factors. In one, pearl oysters were exposed under controlled conditions at different combinations of temperature (24 and 28 degrees C) and food level (10,000 and 40,000 cells mL(-1)); whereas in the other, pearl oysters were conditioned under natural conditions into the lagoon and subjected to successive 17-estradiol injections (100g per injection). Gametogenesis and sex ratio were assessed by histology for each treatment. In parallel, mRNA expressions of nine marker genes of the sexual pathway (pmarg-foxl2, pmarg-c43476, pmarg-c45042, pmarg-c19309, pmarg-c54338, pmarg-vit6, pmarg-zglp1, pmarg-dmrt, and pmarg-fem1-like) were investigated. Maximum maturation was observed in the treatment combining the highest temperature (28 degrees C) and the highest microalgae concentration (40,000 cells mL(-1)), where the female sex tended to be maintained. Injection of 17-estradiol induced a significant increase of undetermined stage proportion 2 weeks after the final injection. These results suggest that gametogenesis and gender in adult pearl oysters can be controlled by environmental factors and estrogens. While there were no significant effects on relative gene expression, the 3-gene-pair expression ratio model of the sexual pathway of P. margaritifera, suggest a probable dominance of genetic sex determinism without excluding a mixed sex determination mode (genetic+environmental).
Plan de classement
Limnologie biologique / Océanographie biologique [034]
Identifiant IRD
PAR00014175
