%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sankar, S. A.
%A Rathored, J.
%A Metidji, S.
%A Lagier, J. C.
%A Khelaifia, S.
%A Labas, N.
%A Musso, D.
%A Raoult, Didier
%A Fournier, P. E.
%T Clostridium polynesiense sp nov., a new member of the human gut microbiota in French Polynesia
%D 2015
%L PAR00014086
%G ENG
%J Anaerobe
%@ 1075-9964
%K Clostridium polynesiense genome ; Culturomics ; Taxono-genomics
%K POLYNESIE FRANCAISE
%M ISI:000367027400014
%P 79-87
%R 10.1016/j.anaerobe.2015.10.004
%U https://www.documentation.ird.fr/hor/PAR00014086
%V 36
%W Horizon (IRD)
%X Strain MS1, a Gram-positive, obligately anaerobic, motile and spore-forming rod belonging to the Clostridium genus, was isolated from the feces of a healthy Polynesian male living in French Polynesia. The temperature range for growth was 30-45 degrees C We sequenced its complete genome and studied its phenotypic characteristics. The 3,560,738-bp long genome (one chromosome, no plasmid, G + C content 34%) contained 3535 protein-coding and 70 RNA genes. Strain MS1 exhibited a 98.24% 16S rRNA similarity with Clostridium amylolyticum, the phylogenetically closest species. When compared with other Clostridium species with standing in nomenclature, it had an average genomic similarity of 68.8-70%, a unique MALDI-TOF spectrum, and differed in nitrate reduction, motility and L-arabinose and D-lactose metabolism with most of the closest species. Therefore, strain MS1 is sufficiently distinct from type strains of the genus Clostridium to represent a novel species within this genus, for which the name Clostridium polynesiense sp. nov. is proposed. The type strain of C polynesiense is MS1(T) (= CSUR P630 = DSM 27072).
%$ 084 ; 054