@article{PAR00013938,
  title = {{A}ssessment of the in vitro antimicrobial activity of {L}actobacillus species for identifying new potential antibiotics},
  author = {{D}ubourg, {G}. and {E}lsawi, {Z}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he bacteriocin-mediated antimicrobial properties of {L}actobacillus spp. have been widely studied, leading to the use of these micro-organisms in the food industry as preservative agents against foodborne pathogens. {I}n an era in which antibiotic resistance is becoming a public health issue, the antimicrobial activity of {L}actobacillus spp. could be used for the discovery of new potential antibiotics. {T}hus, it is essential to have an accurate method of screening the production of antimicrobial agents by prokaryotes. {M}any in vitro assays have been published to date, largely concerning but not limited to {L}actobacillus spp. {H}owever, these methods mainly use the spot-on-the-lawn method, which is prone to contamination during the overlay stage, with protocols using methanol vapours or the reverse side agar technique being applied to avoid such contamination. {I}n this study, a method combining the spot-on-the-lawn and well diffusion methods was tested, permitting clear identification of inhibition zones from eight {L}actobacillus spp. towards clinical isolates of 12 species (11 bacteria and 1 yeast) commonly found in human pathology. {L}actobacillus plantarum {CIP} 106786 and {L}actobacillus rhamnosus {CSUR} {P}567 exhibited the widest antimicrobial activity, whereas {L}actobacillus acidophilus strain {DSM} 20079 was relatively inactive. {I}n addition, the putative {MIC}50 of {L}. rhamnosus against {P}roteus mirabilis was estimated at 1.1 x 10(9) {CFU}/m{L} using culture broth dilution. {I}n conclusion, considering the increasing cultivable bacterial human repertoire, these findings open the way of an effective method to screen in vitro for the production of potential antimicrobial compounds.},
  keywords = {{L}actobacillus ; {S}creening ; {I}n vitro ; {B}acteriocins ; {A}ntibiotics},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {46},
  numero = {5},
  pages = {590--593},
  ISSN = {0924-8579},
  year = {2015},
  DOI = {10.1016/j.inantimicag.2015.05.011},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013938},
}