@article{PAR00013736,
  title = {"{ISA}-{L}ation" of single-stranded positive-sense {RNA} viruses from non-infectious clinical/animal samples},
  author = {{A}ubry, {F}. and {N}ougairede, {A}. and de {F}abritus, {L}. and {P}iorkowski, {G}. and {G}ould, {E}. {A}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{I}solation of viral pathogens from clinical and/or animal samples has traditionally relied on either cell cultures or laboratory animal model systems. {H}owever, virus viability is notoriously susceptible to adverse conditions that may include inappropriate procedures for sample collection, storage temperature, support media and transportation. {U}sing our recently described {ISA} method, we have developed a novel procedure to isolate infectious single-stranded positive-sense {RNA} viruses from clinical or animal samples. {T}his approach, that we have now called "{ISA}-lation", exploits the capacity of viral c{DNA} subgenomic fragments to re-assemble and produce infectious viral {RNA} in susceptible cells. {H}ere, it was successfully used to rescue enterovirus, {C}hikungunya and {T}ick-borne encephalitis viruses from a variety of inactivated animal and human samples. {ISA}-lation represents an effective option to rescue infectious virus from clinical and/or animal samples that may have deteriorated during the collection and storage period, but also potentially overcomes logistic and administrative difficulties generated when complying with current health and safety and biosecurity guidelines associated with shipment of infectious viral material.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {9},
  pages = {e0138703},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0138703},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013736},
}