Publications des scientifiques de l'IRD

Aubry F., Nougairede A., de Fabritus L., Piorkowski G., Gould E. A., de Lamballerie Xavier. (2015). "ISA-Lation" of single-stranded positive-sense RNA viruses from non-infectious clinical/animal samples. Plos One, 10 (9), p. e0138703. ISSN 1932-6203.

Titre du document
"ISA-Lation" of single-stranded positive-sense RNA viruses from non-infectious clinical/animal samples
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000361800700073
Auteurs
Aubry F., Nougairede A., de Fabritus L., Piorkowski G., Gould E. A., de Lamballerie Xavier
Source
Plos One, 2015, 10 (9), p. e0138703 ISSN 1932-6203
Isolation of viral pathogens from clinical and/or animal samples has traditionally relied on either cell cultures or laboratory animal model systems. However, virus viability is notoriously susceptible to adverse conditions that may include inappropriate procedures for sample collection, storage temperature, support media and transportation. Using our recently described ISA method, we have developed a novel procedure to isolate infectious single-stranded positive-sense RNA viruses from clinical or animal samples. This approach, that we have now called "ISA-lation", exploits the capacity of viral cDNA subgenomic fragments to re-assemble and produce infectious viral RNA in susceptible cells. Here, it was successfully used to rescue enterovirus, Chikungunya and Tick-borne encephalitis viruses from a variety of inactivated animal and human samples. ISA-lation represents an effective option to rescue infectious virus from clinical and/or animal samples that may have deteriorated during the collection and storage period, but also potentially overcomes logistic and administrative difficulties generated when complying with current health and safety and biosecurity guidelines associated with shipment of infectious viral material.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Identifiant IRD
PAR00013736
Contact