@article{PAR00013612,
  title = {{E}valuation of the {PREVIA} ({R}) {I}sola automated seeder system compared to reference manual inoculation for antibiotic susceptibility testing by the disk diffusion method},
  author = {{L}e {P}age, {S}. and van {B}elkum, {A}. and {F}ulchiron, {C}. and {H}uguet, {R}. and {R}aoult, {D}idier and {R}olain, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he disk diffusion ({DD}) method remains the most popular manual technique for antibiotic susceptibility testing ({AST}) in clinical microbiology laboratories. {T}his is because of its simplicity, reproducibility, and limited cost compared to (automated) microdilution systems, which are usually less sensitive at detecting certain important mechanisms of resistance. {H}ere, we evaluate the {PREVIA} ({R}) {I}sola automated seeder system using a new protocol for spreading bacterial suspensions (eight deposits of calibrated inocula of bacteria, followed by two rounds of rotation) in comparison with manual {DD} reference testing on a large series of clinical and reference strains. {T}he average time required for seeding one agar plate for {DD} with this new protocol was 51 s per plate, i.e., 70 agar plates/h. {R}eproducibility and repeatability was assessed on three reference and three randomly chosen clinical strains, as usually requested by the {E}uropean {C}ommittee on {A}ntimicrobial {S}usceptibility {T}esting ({EUCAST}), and was excellent compared to the manual method. {T}he standard deviations of zones of growth inhibition showed no statistical discrimination. {T}he correlation between the two methods, assessed using 294 clinical isolates and a panel of six antibiotics (n = 3,528 zones of growth inhibition measured), was excellent, with a correlation coefficient of 0.977. {T}he new {PREVIA} ({R}) {I}sola protocol adapted for {DD} had a sensitivity of 99 % and a specificity of 100 % compared to the manual technique for interpreting {DD} as recommended by the {EUCAST}.},
  keywords = {{EUROPE}},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {34},
  numero = {9},
  pages = {1859--1869},
  ISSN = {0934-9723},
  year = {2015},
  DOI = {10.1007/s10096-015-2424-8},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013612},
}