@article{PAR00013601,
  title = {{I}dentification of tick species and disseminate pathogen using hemolymph by {MALDI}-{TOF} {MS}},
  author = {{Y}ssouf, {A}. and {A}lmeras, {L}. and {B}erenger, {J}. {M}. and {L}aroche, {M}. and {R}aoult, {D}idier and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}atrix {A}ssisted {L}aser {D}esorption/{I}onization {T}ime-of-{F}light {M}ass {S}pectrometry ({MALDI}-{TOF} {MS}) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. {T}he challenges of the present study were to assess {MALDI}-{TOF} {MS} profiling for the both identification of tick species and {R}ickettsia spp. in infected ticks using hemolymph as protein mixture. {M}ethods: {F}irstly, hemolymph protein mixture from legs of 5 tick species, {R}hipicephalus sanguineus, {R}hipicephalus bursa, {D}ermacentor marginatus, {H}yalomma marginatum rufipes and {A}mblyomma variegatum infected by {R}ickettsia africae were submitted to {MALDI}-{TOF} {MS} to assess tick species identification ability. {S}econdly, hemolymph {MS} spectra from {R}h. sanguineus infected or not by {R}ickettsia c. conorii were compared to detect protein profiles changes. {F}inally, leg hemolymph {MS} spectra from new specimens of the 5 tick species were tested blindly including ticks infected by {R}. c. conorii. {D}iscriminating mass peaks distinguishing the {R}. c. conorii infected and non-infected {R}h sanguineus were determined. {R}esults: {C}onsistent and reproducible {MS} profiles were obtained into each tick species. {C}omparison of {MS} spectra revealed distinct hemolymph protein profiles according to tick species. {MS} spectra changes were observed between hemolymphs from {R}. c. conorii-infected and non-infected {R}h. sanguineus specimens, revealing 17 discriminating mass peaks. {C}lustering analysis based on {MS} protein profiles highlighted that hemolymph samples were grouped according to tick species. {A}ll tick hemolymph samples blindly tested against our home-made arthropod {MS} reference database were correctly identified at the species distinguishing also {R}. c. conorii-infected from {R}ickettsia-free {R}h. sanguineus specimens. {C}onclusion: {T}he present study demonstrated the use of hemolymph {MS} profiles for dual identification of tick species and associated pathogens. {T}his concomitant identification could be helpful for tick entomological diagnosis, notably for specimens removed directly on patients.},
  keywords = {{MALDI}-{TOF} mass spectrometry ; {T}icks ; {R}ickettsia ; {E}ntomological diagnosis},
  booktitle = {},
  journal = {{T}icks and {T}ick-{B}orne {D}iseases},
  volume = {6},
  numero = {5},
  pages = {579--586},
  ISSN = {1877-959{X}},
  year = {2015},
  DOI = {10.1016/j.ttbdis.2015.04.013},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013601},
}