%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Asmar, S.
%A Chatellier, S.
%A Mirande, C.
%A van Belkum, A.
%A Canard, I.
%A Raoult, Didier
%A Drancourt, M.
%T A novel solid medium for culturing Mycobacterium tuberculosis isolates from clinical specimens
%D 2015
%L PAR00013497
%G ENG
%J Journal of Clinical Microbiology
%@ 0095-1137
%M ISI:000358290200024
%N 8
%P 2566-2569
%R 10.1128/jcm.01149-15
%U https://www.documentation.ird.fr/hor/PAR00013497
%V 53
%W Horizon (IRD)
%X The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isolates at 10(5), 10(3), and 10 CFU yielded colonies in 5.7 +/- 1.5 days, 7.6 +/- 0.8 days, and 10.8 +/- 1.7 days versus 1.5 +/- 0.4 days, 3.5 +/- 0.6 days, and 4.9 +/- 1 days in MOD9 (P < 0.05, Student's t test). Further, the time to detectable growth of M. tuberculosis was measured on MOD9 and Lowenstein-Jensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. The contamination rate was 1.6% (4/250) on MOD9 versus 4.4% (11/250) on Lowenstein-Jensen medium (P = 0.11, Fisher's exact test). Chlorhexidine-MOD9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-LJ (P = 0.5195, Fisher's exact test). All together, eight M. tuberculosis isolates were cultured solely from chlorhexidine-MOD9, and two M. tuberculosis isolates were cultured solely from chlorhexidine-LJ. The time to detection was 9.8 +/- 3.9 (range, 5 to 18) days for chlorhexidine-MOD9 versus 17.4 +/- 5.9 (range, 10 to 35) days for chlorhexidine-LJ (P < 0.05, Student's t test). These data indicate the superiority of the MOD9 medium over the standard LJ medium following chlorhexidine decontamination for the recovery of M. tuberculosis.
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