@article{PAR00013497,
  title = {{A} novel solid medium for culturing {M}ycobacterium tuberculosis isolates from clinical specimens},
  author = {{A}smar, {S}. and {C}hatellier, {S}. and {M}irande, {C}. and van {B}elkum, {A}. and {C}anard, {I}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative {M}ycobacterium tuberculosis bacteria. {W}e developed and evaluated the performance of {MOD}9, a new blood-free derivative of the {MOD}4 solid medium on which we previously reported for the isolation and culture of mycobacteria. {F}irst, inoculation of {L}owenstein-{J}ensen medium with 21 {M}. tuberculosis isolates at 10(5), 10(3), and 10 {CFU} yielded colonies in 5.7 +/- 1.5 days, 7.6 +/- 0.8 days, and 10.8 +/- 1.7 days versus 1.5 +/- 0.4 days, 3.5 +/- 0.6 days, and 4.9 +/- 1 days in {MOD}9 ({P} < 0.05, {S}tudent's t test). {F}urther, the time to detectable growth of {M}. tuberculosis was measured on {MOD}9 and {L}owenstein-{J}ensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. {T}he contamination rate was 1.6% (4/250) on {MOD}9 versus 4.4% (11/250) on {L}owenstein-{J}ensen medium ({P} = 0.11, {F}isher's exact test). {C}hlorhexidine-{MOD}9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-{LJ} ({P} = 0.5195, {F}isher's exact test). {A}ll together, eight {M}. tuberculosis isolates were cultured solely from chlorhexidine-{MOD}9, and two {M}. tuberculosis isolates were cultured solely from chlorhexidine-{LJ}. {T}he time to detection was 9.8 +/- 3.9 (range, 5 to 18) days for chlorhexidine-{MOD}9 versus 17.4 +/- 5.9 (range, 10 to 35) days for chlorhexidine-{LJ} ({P} < 0.05, {S}tudent's t test). {T}hese data indicate the superiority of the {MOD}9 medium over the standard {LJ} medium following chlorhexidine decontamination for the recovery of {M}. tuberculosis.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {53},
  numero = {8},
  pages = {2566--2569},
  ISSN = {0095-1137},
  year = {2015},
  DOI = {10.1128/jcm.01149-15},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013497},
}